I came across a free, cross-platform, flow cytometry analysis program, called FCSalyzer. Its author is Dr. Sven Mostböck and the program is under active development. I gave it a try and I should say, it is really promising. Although still in alpha stage of development, it provides all the basic functionality one would expect from a flow cytometry analysis software.
I made a SlackBuild for FCSalyzer and submitted it to SBo. A complete list of supported file formats can be found in
/usr/doc/FCSalyzer-0.9.12_alpha/Datafiles.txt. I have used it to analyse data files acquired by Fortessa and Calibur. The program is quite stable and among its current features are:
- dot and density plots
- creating regions and gates
- statistics, with the option to export them as a text file
- quadrants and markers
- …and more!
The program provides useful and intuitive keyboard shortcuts for its key operations, such as:
Ctrl + Dto duplicate a histogram or dor plot
Ctrl + Ffor formatting
Ctrl + Page Downto switch to next data file
Ctrl + Page Upto switch to previous data file
Ctrl + Tto bring up statistics
Here’s an example of my own data, showing density dot plots, gating, histograms, statistics and overlay: