FCSalyzer: flow cytometry analysis
Posted: 2017-01-29 Filed under: academic | Tags: FACS, flow cytometry, flow cytometry on Linux, offline analysis 3 CommentsI came across a free, cross-platform, flow cytometry analysis program, called FCSalyzer. Its author is Dr. Sven Mostböck and the program is under active development. I gave it a try and I should say, it is really promising. Although still in alpha stage of development, it provides all the basic functionality one would expect from a flow cytometry analysis software.
I made a SlackBuild for FCSalyzer and submitted it to SBo. A complete list of supported file formats can be found in /usr/doc/FCSalyzer-0.9.12_alpha/Datafiles.txt. I have used it to analyse data files acquired by Fortessa and Calibur. The program is quite stable and among its current features are:
- dot and density plots
- histograms
- creating regions and gates
- statistics, with the option to export them as a text file
- quadrants and markers
- overlays
- …and more!
The program provides useful and intuitive keyboard shortcuts for its key operations, such as:
Ctrl + Dto duplicate a histogram or dor plotCtrl + Ffor formattingCtrl + Page Downto switch to next data fileCtrl + Page Upto switch to previous data fileCtrl + Tto bring up statistics
Here’s an example of my own data, showing density dot plots, gating, histograms, statistics and overlay:
Great!
slackalaxy.com 
[…] decided to make a ratiometric flow cytoetry analysis in FCSalyzer, since the program is free, cross-platform and easy to use (also, I just like it a lot). Such […]
i used it today for some basic analysis of data files that wouldn’t open on any of the other free softwares
very easy to use – big thank you to the creator of the software and for sharing it for free
i will try out regios/gates/compensation later and update my feedback
I am sure Dr. Mostböck will be happy to hear your feedback, as well. Here’s a discussion about the program on SourceForge:
https://sourceforge.net/p/fcsalyzer/discussion/contact/